منابع مشابه
The unforeseeable hammerhead ribozyme
Despite its small size, the complex behavior of the hammerhead ribozyme keeps surprising us, even more than 20 years after its discovery. Here, we summarize recent developments in the field, in particular the discovery of the first split hammerhead ribozyme.
متن کاملThe hammerhead ribozyme
The hammerhead ribozyme is a small RNA motif consisting of three helices that intersect at a conserved core. When correctly folded, the hammerhead ribozyme stimulates nearly complete cleavage of the phosphodiester chain at a defined internal site to give 2',3'-cyclic and 5'-hydroxy termini. The cleavage rate is approx. 1 min-' at 25 "C, pH 7.5, and increases proportionally with hydroxide ion co...
متن کاملThe ubiquitous hammerhead ribozyme.
The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-) cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three helices, two of which form essential tertiary interactions for fast self-scission under physiological conditions. Originally discovered in subviral plant pathogens, its presence in several eukaryotic genomes has been rep...
متن کاملSelection of hammerhead ribozymes for optimum cleavage of interleukin 6 mRNA.
Four GUC triplets in the coding region of the MRNA of interleukin 6 (IL-6) were examined for their suitabilty to serve as a target for hammerhead ribozome-mediated cleavage. This selection procedure was performed with the intention to downregulate IL-6 production as a potential treatment of those diseases in which IL-6 overexpression is involved. Hammerhead ribozymes and their respective short ...
متن کاملHammerhead ribozyme kinetics.
The hammerhead ribozyme is a small RNA motif that self cleaves at a specific phosphodiester bond to produce 29,39 cyclic phosphate and 59 hydroxyl termini (Hutchins et al+, 1986; Forster & Symons, 1987a)+ The secondary structure of the hammerhead consists of three helices of arbitrary sequence and length (designated I, II, and III) that intersect at 15 nucleotides termed the catalytic core (Fig...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2001
ISSN: 1465-6906
DOI: 10.1186/gb-spotlight-20011102-01